Rapid, convenient, accurate antibody quantification
Whether you are optimising the assessment of your transient expression, accelerating your cell line development programme, or streamlining process development, frequent, accurate and efficient quantification of antibody titre is crucial.
Quickly select stable high-producing clones and optimise process conditions with the agility of highly accurate and convenient antibody quantification using Amperia Redox Electrochemical Detection (RED).
Quickly and accurately quantify antibody titre at your bench or bioreactor
Remove analytical delays when you need a simple antibody titre
Current quantitative methods such as Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) are often limited to centralised analytical laboratories due to their cost and complexity of operation. This can lead to analytical delays and requirements for sample storage.
Our Amperia RED system offers a robust, cost-effective, automated alternative for efficient, accurate antibody titre measurement.
Scatter plot showing the high correlation between Amperia RED and BLI mAb titre measurements. (R²= 0.9, P <0.0001).
Optimise workflows and focus on implementing your cell culture decisions
Streamline decision-making and optimise bioprocess workflows with actionable antibody titre results available conveniently to any team member, at any bench or bioreactor site, direct from unpurified samples.
- Deploy anywhere: optic-free and compact, the Amperia can be deployed wherever you need it most
- Simple-to-use: automated workflow consistency with no specialist training required.
- Rapid results: process up to 64 samples in just 1 hour
- Crude compatible: RED highly sensitive and specific direct detection enables antibody quantification from crude cell culture samples across a broad range (0.01 µg/mL up to 200 µg/mL) saving time on purification or serial dilutions.
- Accurate and precise: excellent data correlation with BLI, SPR in a recent independent study.
- Cost-effective: the all-in-one optic-free system, including built-in computing, means no calibration or maintenance required between runs and makes Amperia accessible to all laboratories. Standard 96-well microplate operation reduces specialised consumable costs.
Simplified Antibody quantification using Amperia RED
An automated ‘dip and measure’ approach uses pre-coated Protein A sensors optimised for capture of antibodies. Ideal for antibody quantification or binding assays using an antibody as the capture molecule. The assay takes place directly on the sensor tip, where the bound analyte triggers an electrical signal, which is read instantaneously by the device.
Protein A
Capture molecule
Antibody
Sample
HRP tagged antibody
Detection reagent
STEP 1
Protein A
A range of pre-coated RED-Sensors for different applications is available. In this application, the Protein A RED-Sensor is selected, enabling Fc-specific antibody capture without the need for sample modification.
STEP 2
Sample capture
The RED-Sensor is dipped into the sample well containing antibody of unknown concentration. Protein A on the sensor surface binds the Fc region of the antibody.
STEP 3
Detection Antibody
The RED-Sensor is transferred into a detection well containing an HRP-tagged detection antibody. This binds to remaining accessible Protein A sites.
STEP 4
Electrochemical Measurement
The RED-Sensor enters a substrate well containing TMB (Tetramethylbenzidine). Instead of measuring colour change, RED detects the redox reaction electrochemically and in real time. The measured signal is inversely proportional to antibody concentration, and quantification is performed using a standard curve.
STEP 5
Rinse & Repeat
After the measurement, sensors regenerate automatically. A new cycle can begin immediately using Steps 2–4. Each Protein A RED-Sensor can be reused for 10 or more quantification iterations.
Quantification of identical control samples by different measurement methods. Each bar represents a single measurement.
Efficient and accurate quantification of human antibodies in Cell Line Development
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Ready to see how Amperia could get your antibody titre answers quickly and accelerate your cell line development and process optimisation?
