SKU: AK-AB-001

For use with the Amperia™ Protein Quantification System

Format: Up to 320 Tests (5 Sensor Strips included)
Assay Format: Inverse occupancy using Protein A capture

Built for consistent, quantitative analysis of human IgG, this kit uses Protein A affinity capture and an inverse occupancy signal format. The design supports accurate performance across a broad dynamic range, even in crude cell culture supernatants.

With strong reproducibility and reduced setup burden, it’s ideal for antibody screening and early-stage process development.

This kit uses a dip-sensor format with Protein A pre-coating. During incubation, antibodies bind via Fc interaction, followed by detection of unoccupied binding sites to generate an inverse signal.

Assay Workflow Schematic. (1) Sensor surface coated with Protein A or Protein G. (2) Antibody (analyte) binds via Fc interaction. (3) Detection molecule binds remaining unoccupied Fc-binding sites, generating an inverse signal.

This assay supports reproducible mAb quantification across high, mid, and low concentration ranges. Performance is maintained in both clarified and crude matrices, ideal for decision-making in clone selection, screening, or upstream development.

Representative standard curves across high, mid, and low ranges (human IgG1).

This kit includes all required reagents and materials for up to 320 antibody quantification tests.

Safety Data Sheets (SDS) are available for each component.

• Regeneration Buffer | Antibody Quantification (A1-8022)
• Wash Buffer (A1-8023)
• Sample Dilution Buffer | Antibody Quantification (A1-8024)
• Detection Reagent | Antibody Quantification (A1-8025)
• Substrate (A1-8026)

SKU: AK-AB-005

For use with the Amperia™ Protein Quantification System

Format: Up to 320 Tests (5 Sensor Strips included)
Assay Format: Inverse occupancy using Protein G capture

Built for consistent, quantitative analysis of human and mouse IgG, this kit uses Protein G affinity capture and an inverse occupancy signal format. The design supports accurate performance across a broad dynamic range, even in crude cell culture supernatants.

With strong reproducibility and reduced setup burden, it is well suited for antibody screening and early-stage process development.

Built for consistent, quantitative analysis of human and mouse IgG, this kit uses Protein G affinity capture and an inverse occupancy signal format. The design supports accurate performance across a broad dynamic range, even in crude cell culture supernatants. With strong reproducibility and reduced setup burden, it is well suited for antibody screening and early-stage process development.

Assay Workflow Schematic. (1) Sensor surface coated with Protein A or Protein G. (2) Antibody (analyte) binds via Fc interaction. (3) Detection molecule binds remaining unoccupied Fc-binding sites, generating an inverse signal.

This assay supports reproducible IgG quantification across multiple concentration ranges. Performance is maintained in both clarified and crude matrices, enabling confident decision-making in clone selection, screening, or upstream development.

Representative standard curves across high, mid, and low ranges for human IgG1, and for mouse IgG subclasses (IgG1, IgG2a, IgG2b).

This kit includes all required reagents and materials for up to 320 antibody quantification tests.

Safety Data Sheets (SDS) are available for each component.

• Regeneration Buffer | Antibody Quantification (A1-8022)
• Wash Buffer (A1-8023)
• Sample Dilution Buffer | Antibody Quantification (A1-8024)
• Detection Reagent | Antibody Quantification (A1-8025)
• Substrate (A1-8026)

SKU: AK-AAV-002

For use with the Amperia™ Protein Quantification System

Format: Up to 40 Tests
Assay Format: Sandwich assay using AAVX-specific capture

This kit enables robust quantification of AAV total capsids across multiple serotypes using a streptavidin–biotin sandwich assay format. Optimised for upstream and in-process applications, it delivers reproducible results even in complex samples such as clarified lysates and crude harvests.

The capture and detection antibodies are based on Thermo Fisher Scientific’s CaptureSelect™ Biotin Anti-AAVX Conjugate and HRP Anti-AAVX Conjugate, supporting reliable and s calable capsid detection across a broad range of vector types.

A biotinylated anti-AAVX antibody is immobilised onto a streptavidin-coated sensor. AAV particles in the sample bind to the capture antibody, and an HRP-conjugated detection antibody binds to the capsid to generate signal.

Assay Workflow Schematic. (1) Sensor surface with streptavidin coating. (2) Capture antibody binding via biotin-streptavidin interaction. (3) AAV capsid captured by the immobilized antibody. (4) Detection antibody (HRP-conjugated) binds the captured capsid, enabling signal generation.

The AAVX assay provides consistent performance across a wide range of serotypes. Combined with integrated software workflows and simplified setup, it supports confident capsid measurement at key development stages.

Representative standard curves for AAV2, AAV5, AAV6, and AAV8.

This kit includes all required reagents and materials for up to 40 AAV total capsid quantification tests.

Safety Data Sheets (SDS) are available for each component:

• CaptureSelect™ Biotin Anti-AAVX Conjugate (A1-8034)
• CaptureSelect™ HRP Anti-AAVX Conjugate (A1-8035)
• Wash Buffer | AAV Quantification (A1-8027)
• Sample Dilution Buffer | AAV Quantification (A1-8028)
• Substrate (A1-8026)

SKU: AK-AAV-003

For use with the Amperia™ Protein Quantification System

Format: Up to 40 Tests
Assay Format: Sandwich assay using AAV9-specific capture

This kit enables accurate quantification of AAV9 total capsids using a streptavidin–biotin sandwich format. Using a similar streptavidin–biotin assay configuration to the AAVX kit, it provides reproducible signal performance across clarified and crude samples, making it suitable for both development and upstream monitoring workflows.

The assay is powered by Thermo Fisher Scientific’s CaptureSelect™ Biotin Anti-AAV9 Conjugate and corresponding HRP-conjugated detection reagent, offering specificity for AAV9 while maintaining workflow compatibility.

AAV9 capsids bind to immobilised biotin–anti-AAV9 antibodies presented on a streptavidin-coated sensor surface. Detection is achieved via HRP-conjugated antibodies targeting the capsid surface.

Assay Workflow Schematic. (1) Sensor surface with streptavidin coating. (2) Capture antibody binding via biotin-streptavidin interaction. (3) AAV capsid captured by the immobilized antibody. (4) Detection antibody (HRP-conjugated) binds the captured capsid, enabling signal generation.

Designed for researchers working with AAV9-based therapies, this kit supports confident capsid measurement in both crude and purified material. Built-in protocol templates and software guidance streamline assay setup and interpretation. 

Representative AAV9 standard curve.

This kit includes all required reagents and materials for up to 40 AAV total capsid quantification tests.

Safety Data Sheets (SDS) are available for each component:

• CaptureSelect™ Biotin Anti-AAV9 Conjugate (A1-8032)
• CaptureSelect™ HRP Anti-AAV9 Conjugate (A1-8033)
• Wash Buffer | AAV Quantification (A1-8027)
• Sample Dilution Buffer | AAV Quantification (A1-8028)
• Substrate (A1-8026)

SKU: AK-HIS-004

For use with: Amperia™ Protein Quantification System

Format: Up to 80 Tests

Assay Format: Premix Competition using anti-His capture

This kit enables rapid and reliable quantification of His-tagged proteins on the Amperia™ platform. It applies a premix competition format incorporating GenScript’s THE™ His Tag Monoclonal Antibody (Mouse) for selective recognition of poly-His sequences commonly used in recombinant protein production.

Compatible with a wide range of expression systems and tag configurations, the assay delivers accurate measurement across both crude lysates and purified preparations. Ready-to-use reagents and preconfigured sensors simplify setup, supporting confident analysis throughout screening, purification, and process development workflows.

The Amperia™ His-tag assay uses a premix competition format, where His-tagged proteins (the analyte) compete with a HRP-labelled His-tagged detection reagent for the same anti-His capture sites. This inverse signal relationship enables accurate quantification across a broad dynamic range, even in crude lysates.

Assay Workflow Schematic
(1) Sensor with streptavidin surface.
(2) Biotinylated anti-His antibody bound to sensor.
(3) Premixed sample and HRP-labelled His-tagged detection reagent applied, enabling signal generation.

Note: In the Tagged Protein Quantification Kit | His-Tag (AK-HIS-004), steps (1) and (2) are pre-completed during manufacturing. Sensors are supplied ready to use, allowing users to begin directly from the sample incubation and measurement step shown in (3).

The His-Tag assay delivers consistent performance across different His-tagged protein types. Combined with straightforward setup and integrated analysis workflows, it supports confident quantification from early screening through process development.

Representative standard curves for His-tagged human IGF-1 and IL-18 Rβ.

This kit provides all necessary reagents and materials for up to 80 His-tagged protein quantification measurements.

Safety Data Sheets (SDS) are available for each component:

• Wash Buffer (A1-8023)
• Substrate (A1-8026)
• Detection Dilution Buffer | His-Tag Quantification (A1-8037)
• Sample Dilution Buffer | His-Tag Quantification (A1-8038)