When quantifying antibodies, selecting the right Fc-binding partner is essential for accuracy and reliability. Protein A and Protein G are commonly used to capture IgG, but they differ in binding specificity across species and isotypes.
What’s the difference?
- Protein A binds strongly to human IgG1, IgG2, and IgG4, making it ideal for human antibody samples.
- Protein G offers broader binding, including mouse IgG1, and is a better choice when working with mouse samples or mixed species.
Which sensor should you use?
Abselion now offers Amperia™ sensors coated with either Protein A or Protein G:
- Protein A sensors: validated for human IgG quantification
- Protein G sensors: validated for human and mouse IgG quantification
Both sensor types are fully compatible with the Amperia™ system and integrate easily into your existing quantification workflows.
Tip: If you’re unsure about your antibody species or isotype, Protein G is often the safer starting point due to its broader reactivity.
Still have questions? Our team is happy to help you choose the best fit for your assay.