His-tag fusion is widely used to simplify recombinant protein purification, but quantifying tagged proteins during expression or purification can be inconsistent and time-consuming with traditional assays.
This use case highlights how the Amperia™ Protein Quantification System enables direct, reliable measurement of His-tagged proteins using anti-His antibody detection. With guided workflows, inverse assay format, and strong matrix tolerance, the platform supports accurate, reproducible quantification across diverse buffer conditions. Amperia™ delivers consistent results throughout protein expression, purification, and process development.
Highlights
- Direct detection of His-tagged proteins in crude or purified samples
- Wide quantification range with high reproducibility
- Compatible with diverse buffer conditions and sample matrices
- Runs on the Amperia™ platform with integrated workflow support
Download The Document