The VIB Nanobody Core in Belgium supports nanobody discovery and development for academic and industrial partners, providing advanced screening and characterisation services for His-tagged proteins.
The team needed a faster and more reproducible way to quantify nanobody expression across large screening panels. Traditional ELISA workflows required multiple manual steps and long incubation times, limiting throughput and delaying data-driven selection.
Using the Amperia™ Protein Quantification System, nanobody samples were analysed directly from crude lysates, delivering quantitative results within the same day. The automated workflow provided accurate, reproducible measurements across a wide concentration range, with minimal hands-on time and no purification required.
Rapid, consistent data enabled the VIB team to prioritise high-yielding clones faster, streamlining nanobody screening and accelerating early development timelines.
Key Takeaways
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Quantification of His-tagged nanobodies directly from crude lysates, with no purification required
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Same-day quantitative results for large screening panels
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Reliable performance across a wide dynamic range of expression levels
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Consistent, reproducible data enabling faster identification of top-producing clones
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Improved throughput and efficiency in nanobody screening workflows
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