Insert the 96-well plate into the holder. Agitation and temperature control are built in!
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Concentrations as low as 1ng/mL or 10pM
Up to 4 parallel measurements
60 samples can be measured in as little as 60 minutes
Compatible with the standard 96-well plate format
Plate loading
Insert the 96-well plate into the holder. Agitation and temperature control are built in!
Sensor loading
Measurements are performed using custom RED-Sensors. Insert up to 4 sensors into the instrument for parallel measurements.
Analysis built-in
Perform data analysis directly with Amperia. Generate standard curves and quantify samples with a few clicks. All data is saved and protected against changes later on.
The entire assay is carried out by moving the RED-Sensors through a sequence of wells in a 96-well plate loaded with the reagents. See for example how RED is deployed to simplify antibody quantification.
Capture molecule
Sample
Detection reagent
Sample capture
The RED-Sensor is dipped into the well with the sample, which in this case contains a Sample Antibody of unknown concentration. These are captured by the Protein A on the surface of the RED-Sensor. Incubation can take as little as 30 seconds.
Detection Antibody
The RED-Sensor is moved into the next well containing the Detection Antibody tagged with Horseradish Peroxidase (HRP). RED is compatible with standard reagents from most vendors. The Detection Antibody binds to the Sample Antibody in as little as 30 seconds. One feature of RED is that the same wells can be re-used again, meaning the well containing the Detection Antibody can be used for multiple iterations of detection.
Capture molecule
Capture antibody
Sample
Detection reagent
Capture antibody
An anti AAV capture antibody is bound to streptavidin once the sensors are dipped into the first set of wells.
Sample capture
AAVs in sample bind to the capture molecule and antibody complex.
Electrochemical measurement
Redox Electrochemical Detection “RED” takes place, whereby the electrochemical interaction between enzyme and substrate is captured an interpreted as a concentration based off of a standard curve. Specifically the conversion of TMB into TMB+ in the presence of HRP and therefore, binded sample.
Optimized for capture of antibodies. Ideal for antibody quantification or binding assays using an antibody as the capture molecule.
Optimised for capturing viral vectors for physical titer readings.
Surface coating with carboxylic acid functional groups. Add molecules to the surface through peptide bonds using EDC/NHS chemistry. The most versatile RED-Sensor, compatible with most assays.
Coming soon
Amperia will be available for sale Spring 2024. Ahead of the official launch, it is available for pioneers and innovators through our trial programme. Install Amperia in your laboratory and test it at your own convenience, without any purchase commitment.
Are you ready for protein research made simple? Speak to us today and register your interest
